First molecular identification of Sarcocystis hirsuta in Iranian beef: A case report
نویسنده
چکیده
Introduction: The main agents of sarcocystosis in cattle as an intermediate host include S. cruzi, S. hominis and S. hirsuta. A sensitive and specific tool such as molecular-based techniques would be necessary to identify the species. Case report: After collection of beef sample from Yazd slaughterhouse, DNA extraction was done with salting out method. The 18SrRNA gene as a specific target gene was used for molecular detection of Sarcocystis spp, then Restriction Fragment Length Polymorphysm (RFLP) analysis identified the speciesusing Rsa and Bfa. Results showed that our designed molecular method could identify S. hirsuta in beef sample. Conclusion: Based on our knowledge, this study indicates the first report of molecular identification of S. hirsuta in Iran. Copyright © 2014, Shahid Sadoughi Uni Med Sci. All rights reserved.
منابع مشابه
First molecular identification of Sarcocystis hirsuta in Iranian beef: A case report
Introduction: The main agents of sarcocystosis in cattle as an intermediate host include S. cruzi, S. hominis and S. hirsuta. A sensitive and specific tool such as molecular-based techniques would be necessary to identify the species. Case report: After collection of beef sample from Yazd slaughterhouse, DNA extraction was done with salting out method. The 18SrRNA gene as a specific target ...
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Background: Sarcocystosis is one of the most widespread parasitic diseases in cattle caused by three main species of Sarcocystis genus. The major aim of this study was to determine the prevalence of Sarcocystis spp. in meat produced in Yazd, central Iran with special reference to species identification, using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). ...
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